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Book Details :
Published on: 1999-12-15
Released on: 2000-03-09
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Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of the entire genome sequence in 1998. The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution. Information on how to obtain, grow, and maintain C. elegans for use as a model system is given in Chapter 4. Chapters 2 and 3 describe the genome project and show how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data. The next chapter gives the essential practical details of transformation and common uses for the technique. Chapter 6 covers reverse genetics and describes strategies for gene inactivation that are known to work in C elegans: epigenetic inactivation and mutational germ line inactivation. Chapter 7 is designed to help the user analyse phenotype by microscopy and includes Normaski, fluorescence, 4-dimensional, and electron microscopy. Techniques for studying the neurobiology of C. elegans are given in chapter 8. Chapter 9 describes the three commonly used approaches for studying gene expression and Chapter 10 deals with the common methods of molecular biology essential for gene characterization. C. elegans is not the ideal organism for biochemical studies, but chapter 11 describes several procedures for producing biochemically useful quantities of pure tissues. The final chapter is about conventional genetics and details the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. Caenorhabditis Elegans: A Practical Approach will therefore provide all the background information necessary for use of C. elegans as a model system. Publications PAPERS. 2016: Stephen R. Adams Mason R. Mackey Ranjan Ramachandra Sakina F. Palida Lemieux Paul Steinbach Eric A. Bushong Margaret T. Butko Ben N.G ... NanoTemper Technologies GmbH: Monolith NT.115 Series The Monolith NT.115 combines the versatility to use of a broad range of fluorophores and fluorescent proteins with all benefits of the MicroScale Thermophoresis ... Maintenance of C. elegans Although C. elegans can be maintained axenically (Avery 1993) it is difficult and the animals grow very slowly. C. elegans is usually grown monoxenically in the ... Zinc finger nuclease - Wikipedia Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can ... 3View System for Image Capture of 3D Ultrastructures ... The 3View system allows high-throughput high-resolution imaging of a wide variety of samples in 3D. 32k x 24k image support and high-performance stages allow ... Syllabus - University of Madras Biochemistry 6 5 3 75 25 100 Part III C: Paper XII: Practical III 4 6 60 40 100 ... Testudo elegans. Carapace. Plastron. ... A Modern Approach by Alejandro G ... Comparing the human and chimpanzee genomes: Searching for ... Genomic approach 1: Narrowing the search to the important differences Using outgroups to define human-specific changes. Noting a difference between the two genome ... Behavior - WormBook Cultivation: C. elegans are generally raised on OP50 bacterial lawns on NGM agar plates. Altering the size of the lawn the type of bacteria the agar ... Imaging Gatan Inc. There are a number of options and technologies available for digital imaging in transmission electron microscopy (TEM) applications today. Traditionally high energy ... Scientific.Net Foundations of Materials Science and Engineering. Foundations of Materials Science and Engineering (FoMSE) is the periodical edition which consists of series of ...
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